Imaging data revealed that methylation of OTX2 is associated with increased functional connectivity between the ventromedial prefrontal cortex and structures of the medial frontal cortex that have been implicated in MDD- such as the paracingulate gyrus, frontal pole, and subcallosal gyrus (Table 1) (Kaufman et al., 2018). This evidence concerns the gene OTX2 and major depressive disorder.