Six canonical pathways; Axonal guidance signaling, FGF signaling, Glioma Signaling, STAT3 signaling, ERK/MAPK and AMPK signaling, which are highly important in astrocytic functions, were predicted to be dysregulated by ZIKV infection by 48 hpi (Figure 4A), with Z-scores exceeding ± 1.96. The gene discussed is STAT3; the disease is Zika virus infectious disease.