A pathway enrichment assay demonstrated that, in addition to the cAMP pathway in which mTORC1 is involved with target genes (Chang et al., 2015), genes related to carbon metabolism in cancer, nicotine addiction, morphine addiction, renin secretion, insulin secretion, dopaminergic synapses, GABAergic synapses, cholinergic synapses, retrograde endocannabinoid signaling, cell adhesion molecules, neuroactive ligand-receptor interaction, and the calcium signaling pathway were among the most enriched categories (Supplementary Figure 4B). This evidence concerns the gene REN and nicotine dependence.