To achieve overexpression of GKRP in insulinoma cells, we constructed the adenovirus (Ad-GKRP) that overexpressed the fusion protein GKRP-RFP (Gckr sequence cloned from tanycytes; Salgado et al., 2014) under the control of human ubiquitin promoter (Supplementary Figure 1). The gene discussed is GCKR; the disease is pancreatic insulinoma.