The endogenous Gckr/Gck ratioshown in Figure 5A indicate that Gckr is 5-folds enriched over Gck in the liver, conversely, in the hypothalamus and tanycytes the expression of Gckr is 5-folds less than Gk and in pancreas Gck was detected but Gckr was absent, for this reason the Gckr/Gck ratio tend to zero Surprisingly, when the Gckr/Gck ratios were evaluated in transduced cultured cells (insulinoma and tanycytes) the relative expression level Gckr were similar to liver (Figure 5B). Here, GCKR is linked to pancreatic insulinoma.