CTSD and neoplasm: To assess whether BRAFV600E-driven melanoma progression requires TFEB S142 phosphorylation, we xenografted NOD/SCID mice with A375 tumors and found that, upon expression of non-phosphorylatable TFEBS142A, or to a lesser extent WT, BRAFV600E-dependent tumor growth was inhibited and tumors exhibited increased autophagy (p62 and LC3-II) and lysosomal (Cathepsin D) functions compared with controls (Fig. 5a–c and Supplementary Fig. 5a).