Using AR-positive prostate cancer cell lines, in which the suppressive activities of ERβ in cell proliferation and MYC expression were validated, we demonstrated that loss of ZFHX3 also increased cell proliferation and MYC expression, and downregulation of MYC was necessary for ZFHX3 to inhibit cell proliferation. The gene discussed is ZFHX3; the disease is prostate carcinoma.