To further confirm the role of lncRNAs in ALI, we performed a study in vitro to investigate whether interventions targeting on lncRNAs plays a role in the protection of ALI, and we used siRNAs on two PCR-verified lncRNAs, ENSMUST00000170214.1 and ENSMUST00000016031.13, and we observed that the interfering of ENSMUST00000170214.1 and ENSMUST00000016031.13 decreased LPS-induced TNF-α and IL-1β production, considering the important role of TNF-α and IL-1β in ALI [32,33], we proposed that therapy targeting lncRNAs may provide novel direction for the treatment of ALI/ARDS. This evidence concerns the gene IL1B and acute respiratory distress syndrome.