Signal pathway analysis found that both cis- and trans-regulated genes are involved in pathways of cytokines and inflammatory response, IL-1 signaling pathway, focal adhesion, and IL-3 signaling pathway, based on previous findings, these pathways are critical for development of ALI [26,28,29], suggesting dysregulated lncRNAs may play a role in ALI/ARDS through such signal pathways enriched by cis- or trans-target genes. Here, IL3 is linked to acute respiratory distress syndrome.