Next, based on a comparison between the qRT-PCR and microarray data, a list of nine up-regulated genes in MT-CHC01R1.5 (BMP2, BRCA1, DHFR, JAG1, MMP1, MSH2, SPOCK2, TYMS, VIM) were selected and further confirmed in another in vitro model established in our group, obtained from an ICC patient resistant to GEM. The gene discussed is VIM; the disease is intrahepatic cholangiocarcinoma.