Next, based on a comparison between the qRT-PCR and microarray data, a list of nine up-regulated genes in MT-CHC01R1.5 (BMP2, BRCA1, DHFR, JAG1, MMP1, MSH2, SPOCK2, TYMS, VIM) were selected and further confirmed in another in vitro model established in our group, obtained from an ICC patient resistant to GEM. This evidence concerns the gene MMP1 and intrahepatic cholangiocarcinoma.