To explore the possible downstream signaling pathways by which PSPH modulates the progression of NSCLC cells, we investigated the effects of PSPH on cell survival, proliferation, invasion, and metastasis‐related signaling molecules in A549 cells after si‐PSPH transfection, including mTOR, FAK, Stat3, AMPK, AKT, and JNK. The gene discussed is PSPH; the disease is non-small cell lung carcinoma.