The results showed that serum SPINK4 had a higher diagnostic value in CRC, with an AUC of 0.919 (0.864–0.973), sensitivity and specificity of 0.886 and 0.900, respectively, and cut-off value of 2.065, which was better than the diagnostic value of serum CEA, CA125, CA153, and CA199. This evidence concerns the gene CEACAM5 and colorectal carcinoma.