TP53 and Ewing sarcoma: In order to investigate the genetic dependencies for these core cohesin genes in cancer cells harboring inactivating STAG2 mutations, lentiviral shRNA transduction using two independent shRNA sequences for each cohesin subunit was performed in four pairs of isogenic STAG2 human cell lines (H4 glioblastoma, 42MGBA glioblastoma, TC-106 Ewing sarcoma, and RPE primary epithelial cells with hTERT overexpression and TP53 depletion).