To further investigate the early molecular events of EGFR signaling pathways induced by Spautin-1, we detected the expression of phospho-MKK4, MKK4, phospho-MEK1/2, MEK1/2, phospho-EGFR (Y1068), phospho-EGFR (Y1173) and total EGFR in PCa cells at different time points after initiation of Spautin-1 treatment. Here, MAP2K4 is linked to posterior cortical atrophy.