To determine whether SGK3 is required for activated PIK3CA mutant induced hepatic steatosis in vivo, we hydrodynamically transfected PIK3CA(H1047R) and PIK3CA(E545K) constructs, which we will refer here to as H1047R and E545K, into the Sgk3+/+ or Sgk3−/− mouse liver. This evidence concerns the gene SGK3 and fatty liver disease.