IFNG and infection: rodentium infection produced IFNγ, IL-17A, and IL-22 (Fig 2A). Quantification revealed that ChAT-GFP+ T-cells express more IFNγ, IL-17A, and IL-22 by MFI (mean fluorescence intensity) compared to ChAT-GFP- T-cells (Fig 2B). Despite this, it is important to note that the frequency of ChAT-GFP+ T-cells actively producing IFNγ and IL-17A were significantly less compared to ChAT-GFP- T-cells.