In line with the in vitro data, the mRNA levels of multiple known TFEB target gens, namely mucolipin‐1 (MCOLN1), hypoxanthine‐guanine phosphoribosyl‐transferase (HPRT), v‐type proton ATPase subunit d1 (ATP6V0D1), PPP3CA and CTSB were rectified by the administration of polydatin in MCD‐diet‐induced NASH in mice. This evidence concerns the gene CTSB and metabolic dysfunction-associated steatohepatitis.