SPHK1 and Familial prostate cancer: Gene-set enrichment analysis of gene ontology biological process enrichment pathways showed that in prostate cancer cells SK1 KD has induced downregulation of pathways linked with DNA conformation change, chromatin assembly and regulation of G1/S transition of mitotic cell cycle and notably enrichment of Wnt signaling pathway, cell motility and MAP kinase activity (Supplementary Table S1, examples of linked pathways shown in Figure 6).