Shank3 heterozygous and knockout rats exhibited increased anxiety behavior, as indicated by the increased amount of time spent in the outer region of the open field compared with wild-type littermates (Shank3+/+: 546.4 ± 6.498 s, n = 13; Shank3+/–: 576.8 ± 3.456 s, n = 18; Shank3−/−: 581.4 ± 9.220 s, n = 10; P < 0.001; Figure 5A). Here, SHANK3 is linked to Anxiety.