We therefore focused on GATA4.Western blot analysis showed that GATA4 expression in human lung cancer cell lines including NCI-H226, NCI-H23, NCI-H460, PC9, and A549 cells was consistently lower than normal lung epithelial cell lines including HSEAC37, HSEAC2KT, and HBEC30 (Fig. 1b). The gene discussed is GATA4; the disease is lung cancer.