The relative abundance of Parabacteroides was negatively correlated with body weight gain (P<0.05), hepatic triglyceride content (P<0.05), epididymal fat (P<0.05), ALT (P<0.05) and leptin (P<0.05) plasma levels in our early obesity and NAFLD model. Here, LEP is linked to obesity due to melanocortin 4 receptor deficiency.