TKT and hepatocellular carcinoma: To further validate the non-metabolic functions of nuclear TKT in HCC cells, a series of TKT overexpressing stable cell lines, including TKT-Y4A (or TKT-K6R), TKT-D155A and TKT-D155A-Y4A (or TKT-D155A-K6R) in Huh7 and HepG2 cells were established (Fig. 6a).