Conversely, Garcia-Cao et al. showed that transgenic overexpression of PTEN in mice decreased the levels of PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3) and glutaminase, key rate-limiting enzymes responsible for glycolysis and glutaminolysis respectively, and two important metabolic features of tumour cell growth [124]. This evidence concerns the gene PFKFB3 and neoplasm.