Pacheco and colleagues [125] performed a genome-wide clustered regularly interspaced short palindromic repeats with Cas9 (CRISPR/Cas9) loss-of-function screening in the colonic epithelial cell line HT-29 to identify mutants with elevated rates of survival following infection with EHEC O157:H7 strain EDL933 ΔespZ. This strain has high levels of T3SS activity and produces both Stx1a and Stx2a. This evidence concerns the gene STX2 and infection.