To gain insight into the biological functional of LSINCT5 on osteosarcoma cells, we observed the LSINCT5 expression levels in osteosarcoma tissues and cell lines (HOS, G-292 and Saos-2), and chose G-292 cells for loss-of-function study and HOS cells for gain-of-function study. This evidence concerns the gene LSINCT5 and osteosarcoma.