In normal tissues, NDGR4 have three major variants—two are preferentially expressed in brain (B and Bvar) and one in heart (H).9 We chose the MCF-7 breast tumor cell line since it expresses high levels of all three NDRG4 protein isoforms (Fig. 4c) and retains several characteristics associated with a poorly aggressive disease.27 This evidence concerns the gene NDRG4 and breast neoplasm.