17 NDRG4 methylation was detected in 16.4% (10/61, by nested MS-PCR) of the samples analyzed and was significantly associated with the number of positive lymph nodes (p = 0.025), TP53 overexpression (p = 0.014) and tumor size (p = 0.036) (Table 1). The gene discussed is NDRG4; the disease is neoplasm.