The DAI parameters (bloody stool, diarrhea, and rectal bleeding), colon measurements, and histopathological index scores revealed a better positive effect of Shogaol in improvement of colitis due to its anti-inflammatory effect than 6-TG, and the IHC analysis also revealed a better effect for Shogaol than 6-TG in activation of CD133 which has a significant role in intestinal regeneration and in regulating intestinal homeostasis. The gene discussed is PROM1; the disease is colitis.