To in-depth study the function of BCAR4 in CSCs, we constructed the stable colorectal cancer cells (HCT-116-pBABE-puro-BCAR4 and HCT-8-pLKO.1-BCAR4) with BCAR4 over-expression or inhibition (Fig. 2a). Here, BCAR4 is linked to colorectal cancer.