To explore the biological function of GTSE1 in breast cancer metastasis, we performed transwell assay in MDA-MA-231 and MDA-MB-468 cells using the RNA interference (RNAi) to knockdown GTSE1 expression, which suggested that GTSE1 depletion significantly restrained the migration and invasion abilities of MDA-MB-231 cells (Fig. 5a and c). The gene discussed is GTSE1; the disease is breast cancer.