Figure 5a,b, shows that PSPEI-PAA nanocomplex treated iDCs have enhanced CD80+/86+ expression compared to PIC-lysate protein mixture treatment. From this, it can be concluded that the presence of poly I:C and lysate protein in the PSPEI-PAA nanocomplex has activated and matured iDCs. Then, the PSPEI-PAA was evaluated for antitumor activity in B16F10 tumor model for 14 days. The antitumor response of PSPEI-PAA was enhanced significantly higher than that of PSPEI-A or antigen along with PIC treated tumor mice (Figure 6a). This evidence concerns the gene CD80 and neoplasm.