Overall, the maturation of DCs by PSPEI-PAA nanocomplex has activated the CD3+CD8+ cytotoxic T cells for the tumor killing and due to unknown reasons the CD3+CD4+FOXP3+ Treg cell population were affected by the PSPEI-PAA nanocomplex administration in the B16F10 tumor mice. This evidence concerns the gene CD8A and neoplasm.