ANXA2 and cancer: In contrast, depletion of PRDX2 by shRNA did not lead to up-regulation of ANXA2 in either MDA-MB-231, MCF7 or HCT-116 cancer cells (Supplementary Materials Figure S7A–C).To investigate if the up-regulation of PRDX2 in HT1080 ANXA2 KO cells was regulated by ROS, we treated these cells with the antioxidant N-acetyl cysteine (NAC).