Immunoblot analysis of tumour lysates showed that tumours developed from stem‐like HepG2SF1 cells had increased expression of AFP, CD133 and ALDH1A1 as well as upregulation of the Akt/mTOR axis and lower expression of pAMPK, AMPK, pACC and ACC (Fig. 8C). The gene discussed is AFP; the disease is neoplasm.