We treated SMMC‐7721 cells and Hep‐3B cells with or without LPS for 48 hours, and then tested the population changes of CD133+CD49f+ cells, which represent the CSCs‐like population of HCC.30 We found that LPS treatment increased CD133+CD49f+ cell populations when compared with the untreated controls (Figure 4A). This evidence concerns the gene PROM1 and hepatocellular carcinoma.