When activated, TLR4 signal participates in a number of tumor processes, like EMT,32 proliferation,33 chemoresistance,34 and metastasis35 etc. Our data show that after LPS treatment, TLR4 was activated to enhance the stemness properties of SMMC‐7721 and Hep‐3B cells, as reflected by increased proportion of CD133+CD49f+ cells, side population, sphere formation capacity as well as the chemoresistance properties. The gene discussed is ITGA6; the disease is neoplasm.