Core regulatory miRNAs of miR-520a, miR-548am, miR-3184, miR-548d, miR-4725, miR-148a, miR-4659a and key regulatory transcriptional factors of MBNL1, SLC26A3, BMP4, ZIC1 and ANKRD7 might provide biological insight into the full repertoire of nCRT response of ESCC. This evidence concerns the gene MBNL1 and esophageal squamous cell carcinoma.