YAP1 and lung adenocarcinoma: To determine whether the decrease in nuclear Yap1 staining resulted in decreased expression of Yap1 transcriptional targets, we isolated protein lysates from GFP+ lung adenocarcinoma cells derived from KrasG12D/+; p53−/−; Rosa26-fGFP mice treated with vehicle control, GNF5 alone, docetaxel alone, or combination therapy and lysates were analyzed by Western blotting for known Yap1-regulated targets.