Our previous study showed that LukS‐PV has the ability to induce apoptosis in AML THP‐1 and HL‐60 cells, to investigate the role of C5aR in leukemia cell apoptosis induced by LukS‐PV, we first measured endogenous C5aR protein levels in three human leukemia cell lines (HL‐60, THP‐1, and Jurkat). The gene discussed is C5AR1; the disease is acute myeloid leukemia.