Our study showed that the protein levels of ATG5 and ATG12 were both decreased when GBCDRlnc1 or PGK1 was downregulated in Dox-resistant gallbladder cancer cells and the effect could be neutralized by PGK1 overexpression, which suggested that ATG5-ATG12 conjugate might be a downstream target of GBCDRlnc1/PGK1 pathway. This evidence concerns the gene ATG5 and gallbladder cancer.