Furthermore, immunohistochemical staining of PGK1, LC3, p62, ATG5 and ATG12 showed that PGK1, ATG5 and ATG12 were dramatically downregulated in Lv-shRNA-GBCDRlnc1 group and confirmed that knockdown of GBCDRlnc1 inhibited the autophagic activity of gallbladder cancer cells in vivo, which is consistent with the aforementioned results in vitro (Fig. 8c). Here, ATG12 is linked to gallbladder cancer.