Interestingly, the results of western blot showed that only the protein levels of ATG5 and ATG12 were both significantly reduced in GBCDRlnc1 or PGK1 knockdown gallbladder cancer drug-resistant cells, while PGK1 overexpression rescued the inhibitory effect of GBCDRlnc1 knockdown on ATG5 and ATG12 expression (Fig. 7g). This evidence concerns the gene PGK1 and gallbladder cancer.