Apart from the APOE variant (rs429358), which had double the effect size in the older half of the cohort (Supplementary Table 2), there were minimal differences in effect sizes in a range of sensitivity analyses, including removing individuals on sleep or depression medication, adjustments for BMI and lifestyle factors, and splitting the cohort by median age (Supplementary Data 3 and Supplementary Methods). This evidence concerns the gene APOE and depressive symptom measurement.