In the current study, we found that Lico A could enhance the nuclear transcription of TFEB and the phosphorylation of AMPK, but it could not exhibit the induction of mTOR in LPS/GlaN-induced ALI, indicating that Lico A-induced autophagy activation may be dependent on the AMPK-TFEB signaling pathway. The gene discussed is TFEB; the disease is acute respiratory distress syndrome.