PKM and colorectal carcinoma: To investigate how B7-H3 regulates aerobic glycolysis in CRC, we analyzed the expression of a spectrum of key glycolysis-related genes, including Glucose transporter 1 (GLUT1), Glucose transporter 4 (GLUT4), Lactate dehydrogenase A (LDHA), Lactate dehydrogenase B (LDHB), HK2, pyruvate kinase M 2 (PKM2), HIF-1α and pyruvate dehydrogenase kinase 1 (PDK1) in B7-H3-overexpressing HCT116, and RKO cells with real-time quantitative PCR (RT-qPCR) (Fig. 2a, b).