Furthermore, we utilized the rhTGF‐β1‐induced EMT cellular model and found that ZEB2‐AS1 was critically involved in EMT as demonstrated that depletion of ZEB2‐AS1 largely abolished the effects of rhTGF‐β1 on EMT markers and cell invasiveness of HNSCC. Here, ZEB2 is linked to head and neck squamous cell carcinoma.