Previous studies have revealed up‐regulated lncRNA ZEB2‐AS1 in bladder, lung and pancreatic cancers and its overexpression associated with malignant clinicopathological features.14, 16, 17 To investigate the biological functions of ZEB2‐AS1 and its significance in HNSCC, we initially measured its expression via qRT‐PCR in 71 pairs of freshly collected HNSCC samples and adjacent non‐tumour mucosa. This evidence concerns the gene ZEB2 and head and neck squamous cell carcinoma.