Thus, we next aimed to determine whether ZEB2‐AS1 has the EMT‐inducing role in HNSCC via the conical TGF‐β1‐inducing EMT model.20, 26 Both ZEB2‐AS1 and ZEB2 were significantly up‐regulated when cells were incubated with rhTGF‐β1 for 48 hours. This evidence concerns the gene TGFB1 and head and neck squamous cell carcinoma.