This finding was in agreement with recent report, wherein TGF‐β1 secreted by cancer‐associated fibroblasts induced EMT and enhanced invasion in bladder cancer cells while depletion of ZEB2‐AS1 reversed TGF‐β1‐induced EMT and invasion.28 Indeed, our in vivo animal experiments offered substantial evidence to support the pro‐proliferative and pro‐metastatic roles of ZEB2‐AS1 in HNSCC. Here, ZEB2 is linked to head and neck squamous cell carcinoma.