Ep‐CAM+/CD44+ and Ep‐CAM+/CD133+ cells happen to be the most studied CSC markers in CRC.14, 18 Higher expression of Ep‐CAM has already been reported in CRC and revealed to have high tumourigenic potential in combination with CD44 expression.14, 28, 29 Our flow cytometry analysis showed significant enrichment of Ep‐CAM+/CD44+ and Ep‐CAM+/CD133+ cells in distal margin after curative surgery. This evidence concerns the gene PROM1 and colorectal carcinoma.