POU5F1 and neoplasm: Studies have shown the regulatory role of Wnt signalling in the expression of CD133 and CD44 in CRC.35, 36 Oct4 and β‐catenin are the two major Wnt pathway genes which are involved in stem cell maintenances.37 In our IHC studies, we observed an increased expression of Oct4 and β‐catenin in tumour and distal margins, whereas normal tissues showed minimal expression (Figure 3G).