NOTCH1 and neoplasm: Finally, we observed SMO, LRP, CSKNK2A2, DVL, TEAD, NOTCH1 and KLF4 mutated genes, which are crucial in the regulation of Wnt, Notch and Hedgehog signalling pathways and thus could be the possible culprit behind the enrichment of CSCs in tumour and distal margin (Figure 3F).