GK and metabolic dysfunction-associated steatotic liver disease: In the present study, both the hyperglycemic GK+/− mice and the hyperlipidemic E3L and E3L.GK+/− mice developed hepatic steatosis, in line with the pathogenesis of NAFLD wherein both metabolic overload and hyperlipidemia contribute to the accumulation of triglycerides and cholesterol in the liver.