CD4 and Dravet syndrome: To assess Treg suppressive activity in DGS and DS patients, Treg suppression assay was performed by testing Tregs obtained from thymus or peripheral blood in an allogenic setting upon incubation with Tconv cells (CD4+CD25−) stimulated in vitro with a pan-T stimulus (anti-CD2, anti-CD3, and anti-CD28-coated beads) (Figures 5H,I).