In contrast to many other dietary NASH models, this WD was also able to produce steatosis-induced fibrosis as shown by Sirius Red staining and hydroxyproline assay (Figure 2C), α-SMA expression (Figure 2D,E), and Col1A1 transcription products (Figure 2E). This evidence concerns the gene COL1A1 and metabolic dysfunction-associated steatohepatitis.