To investigate whether 17-AAG enhances proteasomal or lysosomal degradation in wt p53 and mt p53 AML cells, these were exposed to 17-AAG at different doses for 24 h and then treated with the proteasomal inhibitor MG132 or the macroautophagy inhibitor bafilomycin A1 (Baf A1) for 4 hours before analyzing samples by western blotting. The gene discussed is TP53; the disease is acute myeloid leukemia.