The top five most significantly enriched canonical pathways included IL-1 signaling (ratio = 3.26%, P = 1.47 × 10−6), androgen signaling (ratio = 2.7%, P = 2.81 × 10−6), corticotropin-releasing hormone signaling (ratio = 2.65%, P = 2.97 × 10−6), thrombin signaling (ratio = 1.96%, P = 6.93 × 10−7), and molecular mechanisms of cancer (ratio = 1.27%, P = 6.14 × 10−7) (Additional file 1: File S6). The gene discussed is CRH; the disease is cancer.