Among the 53 genes that are transcriptionally upregulated in FISS as well as in soft-tissue sarcomas (vs. normal tissue) in dogs and humans, we validated at the protein level the upregulation of two genes (BARX1, BarH-like homeobox 1; and FN1, fibronectin 1) in FISS-derived cells vs. skin-derived fibroblasts. This evidence concerns the gene BARHL1 and soft tissue sarcoma.