Gene-level cross-species transcriptome analysis), and/or annotated gene functions suggesting regulatory importance, we selected—from the 241 genes that are consistently (with the same direction of cancer/normal fold-change in both primary tissue and in cultured cells) differentially expressed in FISS vs. normal cells—four FISS-upregulated genes (BARX1, FBN2, MMP13, and LATS1) and four FISS-downregulated genes (BARX2, LEF1, GAPDH, and ITM2B) for targeted validation by quantitative polymerase chain reaction (qPCR). Here, ITM2B is linked to cancer.