The infection rate for Arc-AAV-Esr1 was 92 ± 3% (Figure 5—figure supplement 1, n = 3 Esr1-guide1 96 ± 2%; n = 3 Esr1-guide2 86 ± 2%) and only 34 ± 3% of KNDy neurons expressed ERα post infection (Figure 5a n = 3 Esr1-g1, 38 ± 0.3%; n = 3 Esr1-g2, 30 ± 5%). This evidence concerns the gene ARC and infection.