ESR1 and infection: The infection rate for AVPV-AAV-Esr1 was 81 ± 4% (Figure 3d, Esr1-guide 1 [g1] 82 ± 2%, n = 3; Esr1-guide 2 [g2] 81 ± 8%, n = 3) and only 28 ± 1% of AVPV kisspeptin cells expressed ERα post infection (Figure 3d, n = 3, Esr1-guide1 27 ± 0.4%; n = 3, Esr1-guide2 28 ± 2%).