We found that differentially expressed genes in both the oligo(dT) and RiboZero RNA-seq datasets were strongly enriched for more CTCF binding sites compared with genes that showed similar expression in the two ALL subtypes (chi-square test; P = 3.41e−05 and P = 1.549e−05, respectively; Supplementary Fig. 6), in line with the previous experimental data from a mouse model with reduced CTCF expression29. The gene discussed is CTCF; the disease is acute lymphoblastic leukemia.