VPS33B interacts with NESG1 to modulate EGFR/PI3K/AKT/c-Myc/P53/miR-133a-3p signaling and induce 5-Fluorouracil sensitivity in nasopharyngeal carcinoma, and VPS33B was inhibited by LMP-1 and nicotine. This evidence concerns the gene EGFR and nasopharyngeal carcinoma.