TIA1 and infection: To determine whether SGs form during ZIKV infection, Huh7 cells were infected with the Cambodian isolate (160310) at a multiplicity of infection (MOI) of 5, and the formation of SGs at 24 h postinfection was visualized by immunofluorescence and confocal microscopy using an antibody to detect TIA-1, a protein known to facilitate nucleation of SGs (27).